In the Davis lab we have optimised protocols for imaging the various life stages of Drosophila for improved imaging. We have published several articles detailing these protocols in the different life stages and tissues of interest to us. Details can be found by clicking the links below:
Live cell imaging
Fillet preparation to image neurons in larvae
Macrophage preparation and screening
Embryo collection and mounting
Egg chamber isolation for imaging
Correlative in-resin microscopy
This method, which correlates electron and super-resolution fluorescence microscopy, has been optimised to allow a structural resolution of 40-50nm without the use of chemical fixation Using this approach has allowed us to correlate fluorescently labelled structures to the ultrastructure in the same cell while maintaining a high level of structural preservation. Click here to read the full journal article.
The OMX platform provides unprecedented mechanical and thermal stabilities coupled with a photon budget that is dramatically improved over traditional microscope platforms. These characteristics make the OMX microscope outstanding for fast live cell imaging and super-resolution imaging. Moreover, its open flexible architecture makes it particularly amenable to adding other modes of microscopy to the platform. For more information click here
In collaboration with Micron Oxford we are working on developing a cutting-edge, bespoke, structured-illumination microscope.This will enable us to carry experiments on Drosophila nervous systems, using methods such as electrophysiology, while imaging using structural illumination protocols.
We aim in this project to prototype a relatively cheap, programmable fluorescent microscope that would be available to teaching environments such as primary and secondary schools. For further information on our work on microscopy development click here.
Super-resolution data in 3-D structural illumination (3D-SIM) can be difficult to generate for the non-specialist. In SIMcheck we have developed a suite of plugins for the ImageJ software package that assist the user in avoiding common problems with 3D-SIM. SIMcheck also provides a range of calibration tools and utilities for common image processing tasks. Click here to read the full journal article.
An open-source software developed by the Davis lab and our collaborators to analyse the motility of particles within living cells. This flexible program enables the user to carry out complex analyses and associated statistical tests on the movement of particles and to plot the results of these tests. For more information click here.
Particle Detection and Tracking
Detection and tracking of single fluorescently-labeled particles is challenging due to many complicating factors. One of the key issues is that the low signal that is emitted by single particles can be overwhelmed by inherent noise in the imaging system. We have developed two complementary methods that assist in denoising to aid detection of particles and these particles can then be tracked in an automated way. Click here for further information.