Project Title: Elucidating how the brain develops from stem cells using revolutionary imaging technologies at single molecule and cell resolutions
Supervisor(s) names: Prof. Ilan Davis (Drosophila neurobiology expert), Dr. Alfredo Castello (RNA virus expert) and Prof. Martin Booth (expert optical engineer) Dr. Phillipa Timmins, Aurox (in consultation with the CEO, Non-Academic supervisor)
Department(s)/Organisation(s): Biochemistry, Engineering, Oxford
e-mail: informal enquiries: email@example.com
application enquiries: firstname.lastname@example.org
Tel: 01865 613271 (Ask for Darragh Ennis)
Brief description of project: (This project is supported through the Oxford Interdisciplinary Bioscience Doctoral Training Partnership (DTP) studentship programme. The student recruited to this project will join a cohort of students enrolled in the DTP’s interdisciplinary training programme, and will be able to take full advantage of the training and networking opportunities available through the DTP. For further details please visit www.biodtp.ox.ac.uk.)
Please note: This studentship is only open to UK and EU citizens
Understanding how the brain and its full neuronal complexity develops from a limited number of stem cells is one of the most important questions in modern biology. A number of animal RNA viruses are known to infect the brain and affect neural stem cell behaviour, but their mechanism of infection is still poorly understood. The Davis lab has been using the highly accessible fruit fly model to address these questions by applying novel imaging technologies for direct visualisation of all the individual molecule transcripts in every cell in a whole brain (single molecule FISH), and live cell imaging of a developing explanted brain. The huge number of regulatory genes involved in regulating the stem cells, presents us with a major challenge for understanding normal development and the effects of RNA virus infections: visualising the expression of such a large number of genes and their mutations is not possible with current technologies.
Specific Aims of the Project:
1) Build a bespoke laser free confocal microscope based on the Clarity spinning disc with unprecedented speed and high throughput capacity.
2) To undertake a systematic live analysis of the phenotypes and expression of numerous key factors already identified as required for correct stem cell behaviour.
3) To use this system to track at the single molecule and cell level the early stages of infection of a model arthropod-borne RNA virus (Sindbis) in the insect brain.
For this project we are looking for applicants with an interest in RNA biology, Neuroscience, Viral Biology and Microscopy. It is not necessary to have extensive biology experience and those with experience and/or an interest in Physics, Optics or Engineering are particularly encouraged to apply.
For informal queries about this exciting opportunity please contact Darragh (email@example.com).
For questions about the application process please contact Lorraine Damerell (firstname.lastname@example.org)
We are always interested in hearing from prospective PhD/D Phil students. The lab is currently interested in a range of research areas, using Drosophila as a model, including mRNA localisation, regulation of synaptic plasticity, brain development and advanced imaging techniques. We are committed to training graduate students and foster them in the development of their own ideas. We welcome informal enquiries by email to: email@example.com . Please attach a CV and a short statement explaining your motivation to undertake a PhD.
Be aware that all prospective graduate students will have to formally apply to a competitive graduate studies program before they can join the the Davis Lab.
More information about the DPhil program in the Biochemistry department can be found here
We also host students from the following programs:
- Graduate training programme in Neuroscience
- Chromosome and Developmental Biology
- Interdisciplinary Bioscience Doctoral Training Partnership Programme (DTP)
There are currently no vacancies in the our lab. We do however welcome enquiries for researchers interested in applying for fellowships and other funding to firstname.lastname@example.org
Capsid of the Sindbis virus
smFISH image of Drosophila central brain with stem cells (red) and single RNA molecules (white)